Natural ribozymes can cleave RNA and single-stranded DNA (ssDNA) by transesterification or a blend of hydrolytic and transesterification reactions. Now, ribozymes have been discovered that catalyze the hydrolytic cleavage of ssDNA. Similar ribozymes could potentially replace large, immunogenic, protein-based nucleases in gene therapies.

中文翻译：

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基于 RNA 的可编程 DNA 切割

天然核酶可以通过酯交换反应或水解和酯交换反应的混合来切割 RNA 和单链 DNA (ssDNA)。现在，已经发现核酶可以催化单链DNA的水解切割。类似的核酶有可能取代基因治疗中大型的、免疫原性的、基于蛋白质的核酸酶。