AimTo assess the efficacy of heterologous fibrin biopolymer (HFB) in promoting alveolar bone healing after tooth extraction in rats.Materials and MethodsThe upper right incisors of 48 Wistar rats were extracted. Toothless sockets were filled with HFB (HFBG, n = 24) or blood clot (BCG, n = 24). The tooth extraction sites were subjected to micro‐computed tomography (micro‐CT), histological, histomorphometric and immunohistochemical (for Runt‐related transcription factor 2/Runx2 and tartrate‐resistant acid phosphatase/TRAP) analyses on days 0, 7, 14 and 42 after extraction.ResultsSocket volume remained similar between days 0 and 14 (69 ± 5.4 mm3), except in the BCG on day 14, when it was 10% lower (p = .043). Although the number of Runx2+ osteoblasts was high and similar in both groups (34 × 102 cells/mm2), the HFBG showed lower inflammatory process and osteoclast activity than BCG at 7 days. On day 14, the number of Runx2+ osteoblasts remained high and similar to the previous period in both groups. However, osteoclast activity increased. This increase was 55% lower in the HFBG than BCG. In the BCG, the presence of an inflammatory process and larger and numerous osteoclasts on day 14 led to resorption of the alveolar bone ridge and newly formed bone. On day 42, numbers of Runx2+ osteoblast and TRAP+ osteoclasts decreased dramatically in both groups. Although the BCG exhibited a more mature cortical bone formation, it exhibited a higher socket reduction (28.3 ± 6.67%) and smaller bone volume (37 ± 5.8 mm3) compared with HFBG (socket reduction of 14.8 ± 7.14% and total bone volume of 46 ± 5.4 mm3).ConclusionsHFB effectively suppresses osteoclast activity and reduces alveolar bone resorption compared with blood clot, thus preventing three‐dimensional bone loss, particularly during the early healing period. HFB emerges as a promising biopharmaceutical material for enhancing healing processes after tooth extraction.

中文翻译：

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新型异源纤维蛋白生物聚合物对拔牙后牙槽骨愈合的功效和安全性：一项实验性临床前研究

目的评价异源纤维蛋白生物聚合物(HFB)促进大鼠拔牙后牙槽骨愈合的功效。材料与方法拔除48只Wistar大鼠的右上门牙。无齿套筒中填充有 HFB（HFBG、n= 24) 或血凝块 (BCG,n= 24）。拔牙位点在第 0、7、14 天和拔牙后 42。结果第 0 天和第 14 天期间牙槽窝体积保持相似（69 ± 5.4 mm3），但第 14 天的 BCG 除外，当时降低了 10%（p=.043）。尽管 Runx2+ 成骨细胞的数量很高且两组相似（34 × 102细胞/毫米2），7 天时 HFBG 显示出比 BCG 更低的炎症过程和破骨细胞活性。第14天，两组中Runx2+成骨细胞的数量仍然很高，并且与之前的时期相似。然而，破骨细胞活性增加。 HFBG 的这种增加比 BCG 低 55%。在 BCG 中，第 14 天出现的炎症过程和更大且数量更多的破骨细胞导致牙槽骨嵴和新形成的骨吸收。第 42 天，两组的 Runx2+ 成骨细胞和 TRAP+ 破骨细胞数量均显着减少。尽管 BCG 表现出更成熟的皮质骨形成，但它表现出更高的牙槽窝减少 (28.3 ± 6.67%) 和较小的骨体积 (37 ± 5.8 mm)3）与 HFBG 相比（牙槽窝减少 14.8 ± 7.14%，总骨量为 46 ± 5.4 mm3结论与血凝块相比，HFB 能有效抑制破骨细胞活性，减少牙槽骨吸收，从而防止三维骨丢失，特别是在愈合早期。 HFB 成为一种有前途的生物制药材料，可用于增强拔牙后的愈合过程。