RNA is a key biochemical marker, yet its chemical instability and complex secondary structure hamper its integration into DNA nanotechnology-based sensing platforms. Relying on the denaturation of the native RNA structure using urea, we show that restructured DNA/RNA hybrids can readily be prepared at room temperature. Using solid-state nanopore sensing, we demonstrate that the structures of our DNA/RNA hybrids conform to the design at the single-molecule level. Employing this chemical annealing procedure, we mitigate RNA self-cleavage, enabling the direct detection of restructured RNA molecules for biosensing applications.

中文翻译：

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化学退火重组 RNA 用于纳米孔检测

RNA 是一种关键的生化标记物，但其化学不稳定性和复杂的二级结构阻碍了其集成到基于 DNA 纳米技术的传感平台中。依靠使用尿素使天然 RNA 结构变性，我们表明可以在室温下轻松制备重组的 DNA/RNA 杂合体。使用固态纳米孔传感，我们证明了 DNA/RNA 杂交体的结构符合单分子水平的设计。采用这种化学退火程序，我们减轻了 RNA 自裂解，从而能够直接检测用于生物传感应用的重组 RNA 分子。