The aim of the present study was to investigate whether exposure to pesticides beta-cypermethrin (β-CYP) harms the reproductive capacity of advanced-age female mice. The results evidenced that peri-implantation β-CYP exposure significantly reduced the number of fetuses per advanced-age female in the first litter, and the number and weight of implantation sites. The levels of decidualization markers were significantly reduced in β-CYP-administered advanced-age mice. Lower expression of Pcna, Cdk6, Foxo1, Ki67, and p62 protein and mRNA was found in the decidua of β-CYP-treated advanced-age mice. The levels of Bax, cleaved caspase-3, Lc3a/b, Atg, mTOR, and p-mTOR protein, and the ratio of p-mTOR/mTOR protein expression were clearly downregulated by peri-implantation β-CYP exposure. These results indicated that peri-implantation β-CYP exposure may elevate the decline in reproductive capacity of early pregnant mice in advanced age.

中文翻译：

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高效氯氰菊酯对高龄雌性小鼠生殖能力的影响

本研究的目的是调查接触农药β-氯氰菊酯（β-CYP）是否会损害高龄雌性小鼠的生殖能力。结果证明，着床周围β-CYP暴露显着减少了第一窝高龄雌性的胎儿数量以及着床部位的数量和重量。在给予β-CYP的高龄小鼠中，蜕膜化标志物的水平显着降低。在 β-CYP 处理的高龄小鼠蜕膜中发现 Pcna、Cdk6、Foxo1、Ki67 和 p62 蛋白和 mRNA 的表达较低。 Bax、裂解的 caspase-3、Lc3a/b、Atg、mTOR 和 p-mTOR 蛋白的水平以及 p-mTOR/mTOR 蛋白表达的比率明显因植入周围 β-CYP 暴露而下调。这些结果表明，着床期β-CYP暴露可能会加剧高龄早期妊娠小鼠生殖能力的下降。