Despite being protected by the blood–brain barrier and the blood–cerebrospinal fluid barrier, cerebrospinal fluids can contain contaminants that trigger neurotoxic effects, yet there are few effective methods to analyze metabolites of industrial additives. Here, we established a fast and robust analytical methodology, based on ultra-high performance liquid chromatography interfaced with triple quadrupole mass spectrometry, to quantitatively determine concentrations of metabolites of synthetic antioxidants, organophosphate esters, and phthalate esters in human cerebrospinal fluids. The developed method exhibited highly sensitive and stable instrumental performance, satisfactory recoveries, minimal matrix effects, and trace level detection limits. We identified and quantified thirty industrial additive metabolites in cerebrospinal fluid samples, at 0.58–13 ng/mL total concentrations.

尽管受到血脑屏障和血脑脊液屏障的保护，脑脊液可能含有引发神经毒性作用的污染物，但目前几乎没有有效的方法来分析工业添加剂的代谢物。在这里，我们建立了一种快速、稳健的分析方法，基于超高效液相色谱与三重四极杆质谱联用，定量测定人脑脊液中合成抗氧化剂、有机磷酸酯和邻苯二甲酸酯代谢物的浓度。所开发的方法表现出高灵敏度和稳定的仪器性能、满意的回收率、最小的基质效应和痕量水平的检测限。我们鉴定并定量了脑脊液样本中 30 种工业添加剂代谢物，总浓度为 0.58–13 ng/mL。